Conclusion: The blood donor, a garbage collector, spontaneously returned to the blood bank when developing jaundice. The RBC recipient, also infected by HCV, was undergoing bone marrow transplantation and died from fulminant hepatitis, 26 days after the implicated HAV transfusion. HAV viral load on donor follow-up sample and the platelet recipient was 1.3 and 1.5 × 10 3 IU/ml, respectively. Qualitative PCR was positive on samples from all three individuals and phylogenetic analysis of viruses proved HAV transmission to the two recipients of blood products. Both recipients of red blood cells (R1) and platelet concentrate (R2) from the same implicated donation were HAV IgM-negative and IgG-positive. Serological tests for HAV IgM and IgG were negative on donation sample but positive on follow-up sample, confirming donor's HAV acute infection. His serological and NAT tests were negative for HBV and HCV. Case Report: A 31-year-old male donor accepted for donation of a whole blood unit returned to the blood bank with clinical jaundice 20 days after donation. A sequence from a fragment of 168 bp from the VP1/2A HAV region was used to construct a phylogenetic tree. HAV RNA samples were genotyped by direct sequencing of PCR products. Methods: The RNA of HAV was detected by qualitative nested reverse transcription polymerase chain reaction (nested RT-PCR) and quantified by real-time RT-PCR. Background: This paper describes the transmission of hepatitis A virus (HAV) to two blood recipients from a healthy donor that later presented to the blood bank with jaundice.
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